Recombinant DNA technology, as its name implies, relies on techniques that combine DNA from one source with DNA from another. Typically the DNA of interest (passenger DNA) is combined with another DNA molecule (vector DNA) that serves to ensure its replication and selection . Major obstacles to be overcome for any DNA cloning include purification and isolation of the DNA to be cloned, as well as the identification and selection of the cloned DNA of interest.
go to tutorial - go to conclusion